The extent of growth (indicated the turbidity) of the flask was examined. The flasks were incubated for 2 days at then stored in a refrigerator at for 5 days to stop further growth. The broth was split between three 100 mL conical flasks.įlask A was left flask B was then flask content was filtered through a 0.22 filter before adding to the flask. The broth was swirled until the powder dissolved. Methodology Briefly summarise what you did in the lab.Ī nutrient broth was made up adding 2.5 g of Oxoid Nutrient Broth powder (contains beef extract, peptone, sodium chloride, at pH around 7.5) to 100 mL sterile distilled water in a 250 mL bottle. Two possible sterilisation techniques autoclaving filtration are also tested. The aim of this experiment is to demonstrate that culture media are contaminated naturally (i.e. Related documents Practical - Lab practicals schedule Lab report template 5 Lab report 1 Lab report two BIOC1010 Microbiology (Bioc1010): Exp 3 - Colony Growth Analysis Two Bacterial Lab Report 4 BIOC1010 Related Studylists Microbiology Topics 31 - 96 Microbiology Unknown Bacteria Lab Report- Microbio Preview text Experiment 1 BIOC1010: Introduction to Microbiology Laboratory report: experiment 1 Culture medium preparation Introduction Briefly describe what a culture medium is, why it needs to be sterile and what the aims of the experiment were.Ī culture medium is a liquid or gelatinous substance that contains essential nutrients, to cultivate target microorganisms or tissues, for further purposes.Ī culture medium must be sterilised before use so that no unwanted microorganisms grow, which may contaminate the growing sample.
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